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Publications

Gulick, A. M. (2009) Conformational Dynamics in the Acyl-CoA Synthetases, Adenylation Domains of Non-Ribosomal Peptide Synthetases, and Firefly Luciferase. ACS Chemical Biology 4, 811-827.

Shah, M. B., Ingram-Smith, C., Cooper, L. L., Qu, J., Meng, Y., Smith, K. S., and Gulick, A. M. (2009) The 2.1A crystal structure of an Acyl-CoA synthetase from Methanosarcina acetivorans reveals an alternate acyl binding pocket for small branched acyl substrates. Proteins 77, 685-698.

  • 3ETC for the structure of the unliganded Acyl-Adenylating Enyzmes.

    Wu, R., Reger, A. S., Lu, X., Gulick, A. M., and Dunaway-Mariano, D. (2009) The mechanism of domain alternation in the acyl-adenylate forming ligase superfamily member 4-Chlorobenzoate:CoA ligase." Biochemistry 48, 4115-4125 .

  • 3DLP for the structure of the D402P mutant of CBL.

    Gulick, A. M. (2009) Ironing out a new siderophore synthesis strategy. Nature Chem Bio. 5, 143-144.

    Drake, E. J. and Gulick, A. M. (2008) The three-dimensional structures of Pseudomonas aeruginosa PvcA and PvcB, two proteins involved in the synthesis of 2-isocyano-6,7-dihydroxycoumarin. J Mol Biol 384, 192-205.

  • 3E59 for the structure of PvcA
  • 3EAT for the structure of PvcB

    Reger, A. S., Wu, R., Dunaway-Mariano, D, and Gulick, A. M. (2008) Structural characterizatiaon of a 140(degree) domain movement in the two-step reaction catalyzed by 4-chlorobenzoate:CoA ligase. Biochemistry 47, 8016-8025.

  • 3CW8 for the structure of the CBL bound to the 4-chlorobenzoyl-adenylate (conformation 1)
  • 3CW9 for the structure of the CBL bound to AMP and 4-chlorophenacyl-CoA (conformation 2)

    New

  • See a brief movie illustrating the conformational change in CBAL.

    • Wu, R., Cao, J., Lu, X., Reger, A. S., Gulick, A. M., and Dunaway-Mariano, D. (2008) Mechanism of 4-chlorobenzoate:CoA ligase catalysis. Biochemistry 47, 8026-2039.

    Wu, R., Reger, A. S., Cao, J., Gulick, A. M., and Dunaway-Mariano, D. (2007) Rational redesign of the 4-chlorobenzoate binding site of a 4-chlorobenzoate: Coenzyme A ligase for expanded substrate range. Biochemistry. 46, 14487-14499.

  • 2QVX for the structure of the I303G mutant bound to 3-chlorobenzoate.
  • 2QVY for the structure of the I303G mutant bound to 3,4-dichlorobenzoate.
  • 2QVZ for the structure of the I303A mutant bound to 3-chlorobenzoate.
  • 2QW0 for the structure of the I303A mutant bound to 3,4-dichlorobenzoate.

    Drake, E. J., Cao, J., Qu, J., Shah, M. B., Straubinger, R. M., and Gulick, A. M. (2007) The 1.8A crystal structure of PA2412, an MbtH-like protein from the pyoverdine cluster of Pseudomonas aeruginosa. Journal of Biological Chemistry 282, 20425-20434.

  • 2PST for the structure of PA2412

    Reger, A. R., Carney, J. M., and Gulick, A. M. (2007) Biochemical and crystallographic analysis of substrate binding and conformational changes in acetyl-CoA synthetase. Biochemistry 46, 6536-6546.

  • 2P20 for Acs, R584A Mutation
  • 2P2B for Acs, V386A Mutation
  • 2P2F for Wild-Type Acs bound to Acetate, AMP, and CoA
  • 2P2J for Acs, K609A Mutation
  • 2P2M for Acs, R194A Mutation
  • 2P2Q for Acs, R584E Mutation

    • Sundlov, J. A., Garringer, J. A., Carney, J. M., Reger, A. S., Drake, E. J., Duax, W. L, and Gulick, A. M. (2006) Determination of the crystal structure of EntA, a 2,3-dihydro-2,3-dihydroxybenzoic acid dehydrogenase from E. coli. Acta Cryst D. 62, 734-740.

  • 2FWM for the unliganded EntA structure.

    • Drake, E. J., Nicolai, D. A., and Gulick, A. M. (2006) Structure of the EntB multidomain non-ribosomal peptide synthetase and functional analysis of its interaction with the EntE adenylation domain. Chemistry & Biology 13, 409-419.

  • 2FQ1 for the unliganded EntB structure.

    • Gulick, A. M., Lu, X., and Dunaway-Mariano, D. (2004) The crystal structure of 4-chlorobenzoate:CoA ligase/synthetase in the unliganded and aryl substrate bound states. Biochemistry 43(27), 8670-8679.

  • 1T5D for Chlorobenzoyl-CoA Ligase bound to 4-chlorobenzoate
  • 1T5H for Chlorobenzoyl-CoA Ligase (Synthetase), unliganded, Selenomethionine

    • Gulick, A, M, Starai, V. J., Horswill, A. R., Homick, K. M., and Escalante-Semerena, J. C. (2003) The 1.75 A crystal structure of acetyl CoA synthetase bound to adenosine-5'-propylphosphate and Coenzyme A. Biochemistry 42(10), 2866-2873.

  • 1PG4 for wild-type Acs
  • 1PG3 for the acetylated form of Acs